|
KMID : 0545119930030020115
|
|
Journal of Microbiology and Biotechnology
1993 Volume.3 No. 2 p.115 ~ p.122
|
|
|
P(3HB) Accumulation in Alcaligenes eutrophus H16(ATCC 17699) under Nutrient-Rich Condition and Its Induced Production from Saccharides and Their Derivatives
|
|
SONG, JAE JUN
SHIN, YONG CHUL/YOON, SUNG CHUL
|
|
|
Abstract
|
|
|
|
Poly(3-hydroxybutyrate)(P(3HB)) accumulation under nutrient-rich condition with various amounts of (NH_4)_2SO_4 was systematically investigated. The results of the electron-microscopy and the solvent extraction showed that the P(3HB) accumulation is unavoidable even under nutrient-rich condition. This indicates that in a two-step culture of Alcaligenes eutrophus H16, the researches should be careful in interpreting the data of polyhydroxyalkanoates(PHAs) accumulation in terms of the carbon-source fed in the second step because the two-step culture product contains the P(3HB) produced under nutrient-rich condition. The polyester production capability in a two-step batch culture of A. eutrophus H16(ATCC 17699) was also investigated using various saccharides and their derivatives such as glucose, fructose, gluconic acid, glucaric acid, sorbitol, lactose, galactose, and mannose. The polyesters synthesized were characterized by 500MHz ^1H-NMR spectroscopy, intrinsic viscosity[η] measurement in chloroform and differential scanning calorimetry(DSC). 500MHz ^1H-NMR analysis showed that all polyesters synthesized generally contained 1∼2㏖% of 3HV. Another finding is that the glucose utilization can be increased by changing the autoclaving procedure of the substrate to enhance the P(3HB) production yield up to 46 wt% of P(3HB) in dry cells.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|